Research Catalog

Title

Gene therapy protocols. Vol. 2, Design and characterization of gene transfer vectors / edited by Jeffrey E. LeDoux.

Publication

Totowa, N.J. : Humana Press ; London : Springer [distributor], 2008.

Items in the Library & Off-site

Details

Additional Authors

LeDoux, Joseph E.

Description

xii, 313 pages : illustrations; 25 cm.

Series Statement

Methods in molecular biology ; v. 434

Uniform Title

Methods in molecular biology ; v. 434.

Alternative Title

Design and characterization of gene transfer vectors

Subjects

• Genetic Vectors
• Gene Transfer Techniques
• Genetic Therapy > methods
• Medical protocols
• Gene therapy > Methodology

Note

• Previous ed.: 2002.

Bibliography (note)

• Includes bibliographical references and index.

Contents

1. Purification of Retrovirus Particles Using Heparin Affinity Chromatography / Maria de las Mercedes Segura, Amine Kamen and Alain Garnier -- 2. Scaleable Purification of Adenovirus Vectors / John O. Konz, Lee R. Pitts and Sangeetha L. Sagar -- 3. Quantifying the Titer and Quality of Adenovirus Stocks / Fubao Wang, Bill C. Mathis, Allison Montalvo, Jayanthi J. Wolf and John A. Lewis -- 4. Chromatography-Based Purification of Adeno-Associated Virus / Richard H. Smith, Linda Yang and Robert M. Kotin -- 5. Spectroscopic Methods for the Physical Characterization and Formulation of Nonviral Gene Delivery Systems / Salvador F. Ausar, Sangeeta B. Joshi and C. Russell Middaugh -- 6. Real-Time Multiple Particle Tracking of Gene Nanocarriers in Complex Biological Environments / Samuel K. Lai and Justin Hanes -- 7. Production of Lentiviruses Displaying "Early-Acting" Cytokines for Selective Gene Transfer into Hematopoietic Stem Cells / E. Verhoeyen, D. Negre and F. L. Cosset -- 8. Fiber-modified Adenoviruses for Targeted Gene Therapy / Hongju Wu and David T. Curiel -- 9. PEGylated Adenovirus for Targeted Gene Therapy / Catherine R. O'Riordan and Antonius Song -- 10. Transposon-Based Mutagenesis Generates Diverse Adeno-Associated Viral Libraries with Novel Gene Delivery Properties / James T. Koerber and David V. Schaffer -- 11. Photochemical Enhancement of DNA Delivery by EGF Receptor Targeted Polyplexes / Anette Bonsted, Ernst Wagner, Lina Prasmickaite, Anders Hogset and Kristian Berg -- 12. Reducing the Genotoxic Potential of Retroviral Vectors / Ali Ramezani, Teresa S. Hawley and Robert G. Hawley -- 13. Evaluation of Promoters for Use in Tissue-Specific Gene Delivery / Changyu Zheng and Bruce J. Baum -- 14. Adenovirus-Mediated Transduction of Auto- and Dual-Regulated Transgene Expression in Mammalian Cells / Valeria Gonzalez-Nicolini and Martin Fussenegger -- 15. Regulated Expression of Adenoviral Vectors-Based Gene Therapies: Therapeutic Expression of Toxins and Immune-Modulators / James F. Curtin, Marianela Candolfi, Mariana Puntel, Weidong Xiong, A. K. M. Muhammad, Kurt Kroeger, Sonali Mondkar, Chunyan Liu, Niyati Bondale, Pedro R. Lowenstein and Maria G. Castro -- 16. Liver-Directed Gene Therapy Using the Sleeping Beauty Transposon System / Lalitha R. Belur, R. Scott McIvor and Andrew Wilber -- 17. Generation and Functional Analysis of Zinc Finger Nucleases / Toni Cathomen, David J. Segal, Vincent Brondani and Felix Muller-Lerch -- 18. Conditional Gene Expression and Knockdown Using Lentivirus Vectors Encoding shRNA / Jolanta Szulc and Patrick Aebischer.

ISBN

• 9781603272476 (hbk.)
• 160327247X (hbk.)

OCLC

• ocn187294487
• 187294487
• SCSB-5404920

Owning Institutions

Columbia University Libraries